Linked Life Data

18432997

Source:http://linkedlifedata.com/resource/pubmed/id/18432997

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-24
pubmed:abstractText
This protocol describes methods for monitoring intracellular phosphorylation-dependent signaling events on a single-cell basis. This approach measures cell signaling by treating cells with exogenous stimuli, fixing cells with formaldehyde, permeabilizing with methanol, and then staining with phospho-specific antibodies. Thus, cell signaling states can be determined as a measure of how cells interact with their environment. This method has applications in clinical research as well as mechanistic studies of basic biology. In clinical research, diagnostic or drug efficacy information can be retrieved by discovering how a disease affects the ability of cells to respond to growth factors. Basic scientists can use this technique to analyze signaling events in cell lines and human or murine primary cells, including rare populations, like B1 cells or stem cells. This technique has broad applications to take standard biochemical analysis into primary cells to garner valuable information about signaling events in physiologic settings.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1934-368X
pubmed:author
pubmed:copyrightInfo
(c) 2007 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 8.17
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Single-cell phospho-protein analysis by flow cytometry.
pubmed:affiliation
Stanford University, Stanford, California, USA.
pubmed:publicationType
Journal Article