Linked Life Data

18432902

Source:http://linkedlifedata.com/resource/pubmed/id/18432902

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-24
pubmed:abstractText
Mature T lymphocytes of the CD8 or CD4 classes bear alphabeta T cell receptors (TCR) that are specific for a molecular complex consisting of a major histocompatibility complex class I or II (MHC class I or II) molecule bound to a unique self or foreign peptide. Until recently, methods for monitoring the T cell immune response to a viral or tumor antigen were restricted primarily to functional assays based on limiting dilution analysis, because the lack of specific molecular reagents to identify clonal T cells obviated approaches to identify and enumerate specific T cells. Development of efficient methods to express and refold MHC class I molecules with synthetic peptides coincided with identification of specific protein sequences that provide the substrate for enzymatic biotinylation. This combination has led to the development of a straightforward method for generating synthetic TCR ligands, making them tetravalent to provide increased avidity, and labeling them through a streptavidin moiety with useful fluorescent tags such as fluorescein or phycoerythrin. This unit describes the preparation of MHC class I/peptide tetramers in detail, including bacterial expression and refolding of the MHC class I light chain, beta2-microglobulin (beta2m), as well as the formation of a complex consisting of the MHC class I heavy chain of interest, beta2m, and a chosen peptide.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-368X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 17.3
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
MHC-peptide tetramers to visualize antigen-specific T cells.
pubmed:affiliation
Emory University School of Medicine, Atlanta, Georgia, USA.
pubmed:publicationType
Journal Article, Review