Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-24
pubmed:abstractText
IgG can be purified, as described here, by ammonium sulfate precipitation followed by size-exclusion (SE) chromatography. This is the least expensive option available for purification of antibodies. Protein A- and protein G-affinity chromatography are the fastest methods for purifying antibodies, but they are not effective for all subclasses of rat antibody. A protocol for affinity chromatography using anti-rat antibody is provided for purification of rat antibodies. Ion-exchange (IEX) chromatography is described for purifying intact monoclonal and polyclonal antibodies and antibody fragments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-368X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 2.7
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Purification of immunoglobulin G.
pubmed:affiliation
Lancaster University, Lancaster, United Kingdom.
pubmed:publicationType
Journal Article, Review