Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-24
pubmed:abstractText
Cell surface receptors, such as transferrin receptors and MHC molecules, are internalized into the endocytic pathway and recycled to the plasma membrane. Previous assays used to measure endocytosis and recycling were cumbersome and often required radioactive reagents. This unit describes protocols that employ the combination of a cleavable biotin reagent to label surface molecules and a capture ELISA to detect these molecules allowing for rapid and safe quantitation of cell surface protein expression, endocytosis, and recycling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-368X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 18.7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Biotin labeling and quantitation of cell-surface proteins.
pubmed:affiliation
Indiana University School of Medicine, Indianapolis, Indiana, USA.
pubmed:publicationType
Journal Article