Linked Life Data

18432694

Source:http://linkedlifedata.com/resource/pubmed/id/18432694

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-24
pubmed:abstractText
The protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis. The second is the first step to producing large amounts (milligrams) of plasmid DNA and is also based on alkaline lysis of the bacterial cells. The crude lysate generated in this protocol can be further purified by centrifugation using CsCl/ethidium bromide (CsCl/EtBr) equilibrium density gradients. Three support protocols provide information on how to grow overnight and larger cultures of bacteria, and how to monitor bacterial growth with a spectrophotometer. Other methods, some relying on commercially available ion-exchange columns, are discussed in the commentary.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-368X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 10.3
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Preparation of bacterial plasmid DNA.
pubmed:affiliation
State University of New York, Stony Brook, New York, USA.
pubmed:publicationType
Journal Article, Review