Linked Life Data

18431402

Source:http://linkedlifedata.com/resource/pubmed/id/18431402

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-6-24
pubmed:abstractText
We have previously shown by direct comparison with autoradiographic and biochemical measurements that the L-[1-(11)C]leucine positron emission tomography method provides accurate determinations of regional rates of cerebral protein synthesis (rCPS) and the fraction (lambda) of unlabeled leucine in the precursor pool for protein synthesis derived from arterial plasma. In this study, we examine sensitivity of the method to detect changes in lambda and stability of the method to measure rCPS in the face of these changes. We studied four isoflurane-anesthetized monkeys dynamically scanned with the high resolution research tomograph under control and mild hyperphenylalaninemic conditions. Hyperphenylalaninemia was produced by an infusion of phenylalanine that increased plasma phenylalanine concentrations three- to five-fold. In phenylalanine-infused monkeys, plasma leucine concentrations remained relatively constant, but values of lambda were statistically significantly decreased by 11% to 15%; rCPS was unaffected. Effects on lambda are consistent with competitive inhibition of leucine transport by increased plasma phenylalanine. The effect on lambda shows that competition for the transporter results in a reduction in the fraction of leucine in the precursor pool for protein synthesis coming from plasma. Even under these hyperphenylalaninemic conditions, rCPS remains unchanged due to the compensating increased contribution of leucine from protein degradation to the precursor pool.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-11005872, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-1212213, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-14347277, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-15703696, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-15901791, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-1784331, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-3057507, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-3121647, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-3257762, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-3668544, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-5461364, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-7215387, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-7861158, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-8093014, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-819414, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-8331222, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-869903, http://linkedlifedata.com/resource/pubmed/commentcorrection/18431402-8750601
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1559-7016
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1388-98
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Use of acute hyperphenylalaninemia in rhesus monkeys to examine sensitivity and stability of the L-[1-11C]leucine method for measurement of regional rates of cerebral protein synthesis with PET.
pubmed:affiliation
Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health, Bethesda, Maryland 20892-1298, USA. beebec@intra.nimh.nih.gov
pubmed:publicationType
Journal Article, Evaluation Studies, Research Support, N.I.H., Intramural