| pubmed-article:18431362 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C0011306 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C0167627 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C1336636 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C1824671 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C1999177 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:18431362 | lifeskim:mentions | umls-concept:C1516240 | lld:lifeskim |
| pubmed-article:18431362 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:18431362 | pubmed:dateCreated | 2008-5-26 | lld:pubmed |
| pubmed-article:18431362 | pubmed:abstractText | The effectiveness of the dendritic cell (DC) vaccination protocols that are currently in use could be improved by providing the DCs with a more potent maturation signal. We therefore investigated whether the T-cell stimulatory capacity of human monocyte-derived DCs could be increased by co-electroporation with different combinations of CD40L, CD70, and constitutively active toll-like receptor 4 (caTLR4) encoding mRNA. We show that immature DCs electroporated with CD40L and/or caTLR4 mRNA, but not those electroporated with CD70 mRNA, acquire a mature phenotype along with an enhanced secretion of several cytokines/chemokines. Moreover, these DCs are very potent in inducing naive CD4(+) T cells to differentiate into interferon-gamma (IFN-gamma)-secreting type 1 T helper (Th1) cells. Further, we assessed the capacity of the electroporated DCs to activate naive HLA-A2-restricted MelanA-specific CD8(+) T cells without the addition of any exogenous cytokines. When all three molecules were combined, a >500-fold increase in MelanA-specific CD8(+) T cells was observed when compared with immature DCs, and a >200-fold increase when compared with cytokine cocktail-matured DCs. In correlation, we found a marked increase in cytolytic and IFN-gamma/tumor necrosis factor-alpha (TNF-alpha) secreting CD8(+) T cells. Our data indicate that immature DCs genetically modified to express stimulating molecules can induce tumor antigen-specific T cells in vitro and could prove to be a significant improvement over DCs matured with the methods currently in use. | lld:pubmed |
| pubmed-article:18431362 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18431362 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18431362 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18431362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18431362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18431362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18431362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18431362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18431362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18431362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18431362 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18431362 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:18431362 | pubmed:issn | 1525-0024 | lld:pubmed |
| pubmed-article:18431362 | pubmed:author | pubmed-author:ThielemansKri... | lld:pubmed |
| pubmed-article:18431362 | pubmed:author | pubmed-author:HeirmanCarloC | lld:pubmed |
| pubmed-article:18431362 | pubmed:author | pubmed-author:TuyaertsSandr... | lld:pubmed |
| pubmed-article:18431362 | pubmed:author | pubmed-author:BonehillAudeA | lld:pubmed |
| pubmed-article:18431362 | pubmed:author | pubmed-author:NeynsBartB | lld:pubmed |
| pubmed-article:18431362 | pubmed:author | pubmed-author:BosTomas JTJ | lld:pubmed |
| pubmed-article:18431362 | pubmed:author | pubmed-author:FostierKarelK | lld:pubmed |
| pubmed-article:18431362 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:18431362 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18431362 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:18431362 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18431362 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18431362 | pubmed:pagination | 1170-80 | lld:pubmed |
| pubmed-article:18431362 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:18431362 | pubmed:meshHeading | pubmed-meshheading:18431362... | lld:pubmed |
| pubmed-article:18431362 | pubmed:meshHeading | pubmed-meshheading:18431362... | lld:pubmed |
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| pubmed-article:18431362 | pubmed:meshHeading | pubmed-meshheading:18431362... | lld:pubmed |
| pubmed-article:18431362 | pubmed:meshHeading | pubmed-meshheading:18431362... | lld:pubmed |
| pubmed-article:18431362 | pubmed:meshHeading | pubmed-meshheading:18431362... | lld:pubmed |
| pubmed-article:18431362 | pubmed:meshHeading | pubmed-meshheading:18431362... | lld:pubmed |
| pubmed-article:18431362 | pubmed:meshHeading | pubmed-meshheading:18431362... | lld:pubmed |
| pubmed-article:18431362 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18431362 | pubmed:articleTitle | Enhancing the T-cell stimulatory capacity of human dendritic cells by co-electroporation with CD40L, CD70 and constitutively active TLR4 encoding mRNA. | lld:pubmed |
| pubmed-article:18431362 | pubmed:affiliation | Laboratory of Molecular and Cellular Therapy, Department of Physiology-Immunology, Medical School of the Vrije Universiteit Brussel, Brussels, Belgium. aude.bonehill@vub.ac.be | lld:pubmed |
| pubmed-article:18431362 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18431362 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18431362 | lld:pubmed |
