Linked Life Data

18429313

Source:http://linkedlifedata.com/resource/pubmed/id/18429313

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-22
pubmed:abstractText
This unit describes a method for analyzing protein complexes by chemically cross-linking closely positioned amino acids. The cross-linked products are isolated by SDS-PAGE and the bands of interest are excised. Proteins in the excised gel piece are digested with trypsin and the resulting peptides recovered. Chemically bonded peptides are distinct from the multitude of unmodified single peptides by the presence of two carboxy-terminal ends. The protocol also describes incorporation of (18)O at the carboxy-terminal ends and purification and preparation of the peptide mixture for mass spectroscopy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1934-3663
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 19.16
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Determination of protein contacts by chemical cross-linking with EDC and mass spectrometry.
pubmed:affiliation
National Institutes of Health, Bethesda, Maryland, USA.
pubmed:publicationType
Journal Article