Linked Life Data

18429260

Source:http://linkedlifedata.com/resource/pubmed/id/18429260

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-22
pubmed:abstractText
This unit describes basic protocols for efficient and reproducible protein solubilization from a variety of biological samples, including cultured animal cells and tissues, plant cells and tissues, bacteria, nuclei, other subcellular organelles, plasma, serum, and other biological fluids. The optimized extraction process is strongly sample-dependent and cannot be described for every type of sample. Instead, typical protocols are provided as general guidelines and illustrate good starting points for sample-preparation optimization. These solubilization procedures take into account the constraints brought by two-dimensional electrophoresis and are thus well suited for proteomic approaches.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1934-3663
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 22.4
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Preparing protein extracts for quantitative two-dimensional gel comparison.
pubmed:affiliation
Départment Résponse et Dynamique, Cellulaire/BioEnergetique Cellulaire et Pathologique, Commissariat à l'Energie Atomique, Grenoble, France.
pubmed:publicationType
Journal Article