Linked Life Data

18429179

Source:http://linkedlifedata.com/resource/pubmed/id/18429179

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-22
pubmed:abstractText
This unit first describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector to generate a recombinant virus. Methods are also presented for purifying vaccinia virus and for isolating viral DNA, which can be used during transfection. Also presented are selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-3663
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit5.13
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Generation of recombinant vaccinia viruses.
pubmed:affiliation
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
pubmed:publicationType
Journal Article