Linked Life Data

18429092

Source:http://linkedlifedata.com/resource/pubmed/id/18429092

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-22
pubmed:abstractText
Immunoblotting (often referred to as western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. Following electrophoretic separation of proteins and transfer from the gel to an appropriate membrane, the immobilized proteins are probed with specific antibodies to identify and quantitate any antigens present. After being probed with primary antibody, the membrane is washed and the antibody-antigen complexes are identified using horseradish peroxidase (HRPO) or alkaline phosphatase (AP) enzymes coupled to the secondary anti-immunoglobulin-G (anti-IgG) antibody (e.g., goat anti-rabbit IgG). As described in this unit, the detection enzymes are attached directly or via an avidin-biotin bridge to the secondary antibody. Chromogenic or luminescent substrates are also described for visualizing the activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-3663
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 10.10
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Immunoblot detection.
pubmed:affiliation
Hoefer Scientific Instruments, San Francisco, California, USA.
pubmed:publicationType
Journal Article