| pubmed-article:18428918 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18428918 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:18428918 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:18428918 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:18428918 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:18428918 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:18428918 | lifeskim:mentions | umls-concept:C0072667 | lld:lifeskim |
| pubmed-article:18428918 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:18428918 | pubmed:dateCreated | 2008-4-22 | lld:pubmed |
| pubmed-article:18428918 | pubmed:abstractText | Pyrene is a useful chromophore for monitoring the tertiary structure and folding of large RNAs. This unit describes the general preparation of a large RNA (>80 nucleotides in length) that has been site-specifically modified with pyrene at the 2'-position of an individual internal nucleotide. A protocol is provided for derivatizing a 2'-amino-RNA oligonucleotide with a suitably activated pyrene reagent. This pyrene-labeled oligonucleotide is then assembled with other RNA(s) either by covalent ligation or by noncovalent hybridization to form a full-length structured RNA, which may then be studied by equilibrium and stopped-flow fluorescence spectroscopy. | lld:pubmed |
| pubmed-article:18428918 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18428918 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18428918 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18428918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18428918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18428918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18428918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18428918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18428918 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18428918 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:18428918 | pubmed:issn | 1934-9289 | lld:pubmed |
| pubmed-article:18428918 | pubmed:author | pubmed-author:SilvermanScot... | lld:pubmed |
| pubmed-article:18428918 | pubmed:author | pubmed-author:SmalleyMary... | lld:pubmed |
| pubmed-article:18428918 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18428918 | pubmed:volume | Chapter 11 | lld:pubmed |
| pubmed-article:18428918 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18428918 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18428918 | pubmed:pagination | Unit 11.11 | lld:pubmed |
| pubmed-article:18428918 | pubmed:meshHeading | pubmed-meshheading:18428918... | lld:pubmed |
| pubmed-article:18428918 | pubmed:meshHeading | pubmed-meshheading:18428918... | lld:pubmed |
| pubmed-article:18428918 | pubmed:meshHeading | pubmed-meshheading:18428918... | lld:pubmed |
| pubmed-article:18428918 | pubmed:meshHeading | pubmed-meshheading:18428918... | lld:pubmed |
| pubmed-article:18428918 | pubmed:meshHeading | pubmed-meshheading:18428918... | lld:pubmed |
| pubmed-article:18428918 | pubmed:meshHeading | pubmed-meshheading:18428918... | lld:pubmed |
| pubmed-article:18428918 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:18428918 | pubmed:articleTitle | Site-specific fluorescent labeling of large RNAs with pyrene. | lld:pubmed |
| pubmed-article:18428918 | pubmed:affiliation | University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. | lld:pubmed |
| pubmed-article:18428918 | pubmed:publicationType | Journal Article | lld:pubmed |
