Linked Life Data

18428918

Source:http://linkedlifedata.com/resource/pubmed/id/18428918

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-22
pubmed:abstractText
Pyrene is a useful chromophore for monitoring the tertiary structure and folding of large RNAs. This unit describes the general preparation of a large RNA (>80 nucleotides in length) that has been site-specifically modified with pyrene at the 2'-position of an individual internal nucleotide. A protocol is provided for derivatizing a 2'-amino-RNA oligonucleotide with a suitably activated pyrene reagent. This pyrene-labeled oligonucleotide is then assembled with other RNA(s) either by covalent ligation or by noncovalent hybridization to form a full-length structured RNA, which may then be studied by equilibrium and stopped-flow fluorescence spectroscopy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1934-9289
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 11.11
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Site-specific fluorescent labeling of large RNAs with pyrene.
pubmed:affiliation
University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
pubmed:publicationType
Journal Article