Linked Life Data

18428656

Source:http://linkedlifedata.com/resource/pubmed/id/18428656

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-22
pubmed:abstractText
Alphaviral vectors based on Semliki Forest virus and Sindbis virus infect many host cell types, causing rapid and high-level transgene expression. Compared to other viruses used to infect CNS cell and tissue preparations, Semliki Forest virus and Sindbis virus exhibit an outstanding preference for neurons rather than glial cells. High-titer vector generation typically requires biosafety level 1 or 2 containment and occurs in less than 2 days. Wild-type vectors are cytotoxic, permitting short-term transgene expression. However, mutant vectors with decreased cytotoxicity, to prolong host cell survival, have been developed. They also increase transgene expression and cellular coinfection, permitting heteromeric protein expression in individual cells. Other mutants with temperature-dependent control of transgene expression and altered host cell preference to target interneurons and astrocytes rather than principal neurons are available. Because of these advantages, alphaviral vectors are increasingly used in neurobiological and other studies, including structural biology, vaccine development, and cancer treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1934-8576
pubmed:author
pubmed:copyrightInfo
Copyright 2007 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 4.22
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Alphaviruses: Semliki Forest virus and Sindbis virus vectors for gene transfer into neurons.
pubmed:affiliation
Kantonsschule Hohe Promenade, Zurich, Switzerland.
pubmed:publicationType
Journal Article