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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2008-4-22
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pubmed:abstractText |
This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1934-8258
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
Chapter 5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
Unit 5.12
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pubmed:meshHeading |
pubmed-meshheading:18428286-Animals,
pubmed-meshheading:18428286-Cells, Cultured,
pubmed-meshheading:18428286-Chromosomes, Artificial, Bacterial,
pubmed-meshheading:18428286-Chromosomes, Artificial, Yeast,
pubmed-meshheading:18428286-DNA, Recombinant,
pubmed-meshheading:18428286-DNA Primers,
pubmed-meshheading:18428286-Embryo, Mammalian,
pubmed-meshheading:18428286-Gene Transfer Techniques,
pubmed-meshheading:18428286-Genetic Markers,
pubmed-meshheading:18428286-Genetics, Medical,
pubmed-meshheading:18428286-Humans,
pubmed-meshheading:18428286-Membrane Fusion,
pubmed-meshheading:18428286-Mice,
pubmed-meshheading:18428286-Microinjections,
pubmed-meshheading:18428286-Plasmids,
pubmed-meshheading:18428286-Polymerase Chain Reaction,
pubmed-meshheading:18428286-Spheroplasts
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pubmed:year |
2001
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pubmed:articleTitle |
Introduction of large insert DNA into mammalian cells and embryos.
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pubmed:affiliation |
Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
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pubmed:publicationType |
Journal Article
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