Linked Life Data

18428269

Source:http://linkedlifedata.com/resource/pubmed/id/18428269

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-22
pubmed:abstractText
Two protocols discuss labeling PCR products with radioactive labels. The PCR products are then analyzed on a denaturing polyacrylamide gel and visualized by direct autoradiography. The resulting band pattern is used to define the SSLP genotype of the individual. Another method of genotyping uses a simple silver staining technique to detect PCR-amplified SSLPs electrophoresed in denaturing polyacrylamide gels. In a fourth protocol, multiple unlabeled PCR products from one individual are pooled, separated on a denaturing polyacrylamide gel, transferred to a nylon membrane, and sequentially hybridized to nonradioactive end-labeled probes derived from the primers used to amplify each SSLP marker. The probes are detected on film using chemiluminescence. Support protocols describe the preparation of an M13 sequence ladder size standard and digoxigenin labeling of the probe, both of which are required for the chemiluminescent method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-8258
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 2.5
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
PCR methods of genotyping.
pubmed:affiliation
Whitehead Institute, Cambridge, Massachusetts, USA.
pubmed:publicationType
Journal Article