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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2008-5-26
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pubmed:abstractText |
The precursor of the rat mitochondrial flavoenzyme dimethylglycine dehydrogenase (Me(2)GlyDH) has been produced in Escherichia coli as a C-terminally 6-His-tagged fusion protein, purified by one-step affinity chromatography and identified by ESI-MS/MS. It was correctly processed into its mature form upon incubation with solubilized rat liver mitoplasts. The purified precursor was mainly in its apo-form as demonstrated by immunological and fluorimetric detection of covalently bound flavin adenine dinucleotide (FAD). Results described here definitively demonstrate that: (i) covalent attachment of FAD to Me(2)GlyDH apoenzyme can proceed in vitro autocatalytically, without third reactants; (ii) the removal of mitochondrial presequence by mitochondrial processing peptidase is not required for covalent autoflavinylation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0141-8130
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
455-62
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pubmed:meshHeading |
pubmed-meshheading:18423846-Amino Acid Sequence,
pubmed-meshheading:18423846-Animals,
pubmed-meshheading:18423846-Catalysis,
pubmed-meshheading:18423846-Cloning, Molecular,
pubmed-meshheading:18423846-Dimethylglycine Dehydrogenase,
pubmed-meshheading:18423846-Flavin-Adenine Dinucleotide,
pubmed-meshheading:18423846-Gene Expression,
pubmed-meshheading:18423846-Mass Spectrometry,
pubmed-meshheading:18423846-Mitochondria,
pubmed-meshheading:18423846-Mitochondrial Proteins,
pubmed-meshheading:18423846-Molecular Sequence Data,
pubmed-meshheading:18423846-Rats,
pubmed-meshheading:18423846-Recombinant Proteins,
pubmed-meshheading:18423846-Spectrophotometry
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pubmed:year |
2008
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pubmed:articleTitle |
The purified recombinant precursor of rat mitochondrial dimethylglycine dehydrogenase binds FAD via an autocatalytic reaction.
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pubmed:affiliation |
Dipartimento di Biochimica e Biologia Molecolare E. Quagliariello, Università degli Studi di Bari, Via Orabona 4, 70126 Bari, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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