Linked Life Data

18423639

Source:http://linkedlifedata.com/resource/pubmed/id/18423639

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-5-26
pubmed:abstractText
Phenothiazine and derivatives were tested for inhibition of SARS-CoV replication. Phenothiazine slightly inhibited SARS-CoV replication in a neutral red (NR) uptake assay. Adding a propylamino group to give promazine reduced virus yields (VYR assay) with an EC(90)=8.3+/-2.8 microM, but without selectivity. Various substitutions in the basic phenothiazine structure did not promote efficacy. Phenazine ethosulfate was the most potent compound by VYR assay (EC(90)=6.1+/-4.3 microM). All compounds were toxic (IC(50)=6.6-74.5 microM) except for phenoxathiin (IC(50)=858+/-208 microM) and 10-(alpha-diethylamino-propionyl) phenothiazine.HCl (IC(50)=195+/-71.2 microM). Consequently, none were selective inhibitors of SARS-CoV replication (SI values <1-3.3 microM). These data portended the poor efficacy of promazine in a SARS-CoV mouse lung replication model. Intraperitoneal treatment with promazine using a prophylactic (-4h)/therapeutic regimen of 1, 10, or 50mg/(kg day) did not reduce virus lung titers at day 3, yet prolonged virus replication to 14 days. Similar therapeutic promazine doses were not efficacious. Thus, promazine did not affect SARS-CoV replication in vitro or in vivo, nor were any other phenothiazines efficacious in reducing virus replication. Therefore, treating SARS infections with compounds like promazine is not warranted.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-12711465, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-12748632, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-12883457, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-12928031, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-15016880, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-15105133, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-15110833, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-15356152, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-15504263, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-15667124, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-15695582, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-15878786, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-16212898, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-16339739, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-16501078, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-16597209, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-16621037, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-16830004, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-2084068, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-5294431, http://linkedlifedata.com/resource/pubmed/commentcorrection/18423639-9107383
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0166-3542
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
105-13
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Is the anti-psychotic, 10-(3-(dimethylamino)propyl)phenothiazine (promazine), a potential drug with which to treat SARS infections? Lack of efficacy of promazine on SARS-CoV replication in a mouse model.
pubmed:affiliation
Institute for Antiviral Research, Utah State University, 5600 Old Main Hill, Logan, UT 84322-5600, USA. dale.barnard@usu.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural