Linked Life Data

18419298

Source:http://linkedlifedata.com/resource/pubmed/id/18419298

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-18
pubmed:abstractText
Cultured circadian oscillators from peripheral tissues were recently shown to be both cell-autonomous and self-sustained. Therefore, the dominant cause for amplitude reduction observed in bioluminescence recordings of cultured fibroblasts is desynchronization, rather than the damping of individual oscillators. Here, we review a generic model for quantifying luminescence signals from biochemical oscillators, based on noisy-phase oscillators. Our model incorporates three essential features of circadian clocks: the stability of the limit cycle, fluctuations, and intercellular coupling. The model is then used to analyze bioluminescence recordings from immortalized and primary fibroblasts. Fits to population recordings allow simultaneous estimation of the stability of the limit cycle (or equivalently, the stiffness of individual frequencies), the period dispersion, and the interaction strength between cells. Consistent with other work, coupling is found to be weak and insufficient to synchronize cells. Interestingly, we find that frequency fluctuations remain correlated for longer periods than one clock cycle, which is confirmed from individual cell recordings. We discuss briefly how to link the generic model with more microscopic models, which suggests mechanisms by which circadian oscillators resist fluctuations and maintain accurate timing in the periphery.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0091-7451
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
405-11
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Stochastic phase oscillators and circadian bioluminescence recordings.
pubmed:affiliation
Swiss Institute of Bioinformatics, CH-1015 Lausanne, Switzerland.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't