Source:http://linkedlifedata.com/resource/pubmed/id/18418396
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2008-6-4
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| pubmed:abstractText |
Coccidioides posadasii spherules stimulate macrophages to make cytokines via TLR-2 and Dectin-1. We used formalin-killed spherules and 1,3-beta-glucan purified from spherules to stimulate elicited peritoneal macrophages and myeloid dendritic cells (mDCs) from susceptible (C57BL/6) and resistant (DBA/2) mouse strains. DBA/2 macrophages produced more TNF-alpha and IL-6 than macrophages from C57BL/6 mice, and the amount of TNF-alpha made was dependent on both TLR2 and Dectin-1. DCs from C57BL/6 mice made more IL-10 and less IL-23p19 and IL-12p70 than did DBA/2 DC. These responses were inhibited by a monoclonal antibody to Dectin-1. DBA/2 mice expressed full-length Dectin-1, whereas C57BL/6 mice spliced out exon 3, which encodes most of the stalk. RAW cells transduced to express the full-length Dectin-1 responded better to FKS than cells expressing truncated Dectin-1. We compared the isoform of Dectin-1 expressed by 34 C57BL/6 X DBA/2 recombinant inbred (BXD RI) lines with their susceptibility to Coccidioides immitis. In 25 of 34 RI lines susceptibility or resistance corresponded to short or full-length isoforms, respectively. These results suggest that alternative splicing of the Dectin-1 gene contributes to susceptibility of C57BL/6 mice to coccidioidomycosis, and affects the cytokine responses of macrophages and mDCs to spherules.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/dectin 1
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1476-5470
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
9
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
338-48
|
| pubmed:dateRevised |
2011-9-26
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| pubmed:meshHeading |
pubmed-meshheading:18418396-Alternative Splicing,
pubmed-meshheading:18418396-Animals,
pubmed-meshheading:18418396-Cell Line,
pubmed-meshheading:18418396-Coccidioides,
pubmed-meshheading:18418396-Coccidioidomycosis,
pubmed-meshheading:18418396-Dendritic Cells,
pubmed-meshheading:18418396-Gene Expression,
pubmed-meshheading:18418396-Genetic Predisposition to Disease,
pubmed-meshheading:18418396-Immunity, Innate,
pubmed-meshheading:18418396-Interleukin-10,
pubmed-meshheading:18418396-Macrophages, Peritoneal,
pubmed-meshheading:18418396-Membrane Proteins,
pubmed-meshheading:18418396-Mice,
pubmed-meshheading:18418396-Mice, Inbred C57BL,
pubmed-meshheading:18418396-Mice, Inbred DBA,
pubmed-meshheading:18418396-Nerve Tissue Proteins,
pubmed-meshheading:18418396-Protein Isoforms,
pubmed-meshheading:18418396-Species Specificity,
pubmed-meshheading:18418396-Tumor Necrosis Factor-alpha
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| pubmed:year |
2008
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| pubmed:articleTitle |
Susceptibility to Coccidioides species in C57BL/6 mice is associated with expression of a truncated splice variant of Dectin-1 (Clec7a).
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| pubmed:affiliation |
Research Service, VA San Diego Healthcare, San Diego, CA 92161, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|