Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-4-16
pubmed:abstractText
During transcription termination by RNA polymerase II on protein-coding genes, the nuclear 5' exonuclease Rat1/Xrn2 degrades the nascent transcript downstream from the polyadenylation site and "torpedoes" the polymerase. We report that the activity of Rat1 is also required for efficient termination by RNA polymerase I (Pol I) on the rDNA. In strains lacking catalytically active Rat1 or its cofactor Rai1, Pol I reads through the major, "Reb1-dependent" terminator (T1) but stops downstream at the "fail-safe" terminator (T2) and replication fork barrier (RFB). The absence of both Rat1 and the RFB-binding protein Fob1 increased Pol I read-through of T2 and the RFB. We propose that cotranscriptional cleavage of the pre-rRNA by the endonuclease Rnt1 generates a loading site for the Rat1/Rai1 complex, which then degrades the nascent transcript. When Rat1 catches Pol I, which is predicted to be paused at T1, transcription is terminated.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-10411134, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-10523625, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-10589839, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-10805743, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-11142370, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-11290703, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-12504018, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-12783853, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-1425596, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-15073335, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-15314641, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-15337846, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-15489292, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-15565157, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-15565158, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-16043506, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-1628825, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-16319194, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-16598041, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-16648491, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-16651651, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-17043313, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-17259992, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-17603014, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-17639083, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-17991894, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-18007593, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-18280237, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-2685755, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-2689870, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-2714650, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-7515008, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-7608167, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-8057832, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-8417359, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-8602511, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-8663252, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9169440, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9218804, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9237683, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9267035, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9315672, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9433127, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9488433, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9571034, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413717-9717241
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1069-81
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Efficient termination of transcription by RNA polymerase I requires the 5' exonuclease Rat1 in yeast.
pubmed:affiliation
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't