Linked Life Data

18413244

Source:http://linkedlifedata.com/resource/pubmed/id/18413244

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-16
pubmed:abstractText
Lipid droplets are the major intracellular store of lipids in eukaryotic cells. Understanding lipid storage and regulated mobilization of lipids from lipid droplets is essential for understanding the syndromes and diseases associated with excess lipid accumulation. Lipid droplets have been traditionally considered relatively inert structures. However, in recent years it has become apparent that lipid droplets are highly dynamic regulated organelles, which show complex interactions with other cellular compartments. The cellular components involved in regulation of lipid accumulation and release from lipid droplets, and in mediating the complex interactions with other organelles, are only now starting to be unraveled. A particularly important family of proteins in this respect is the Rab GTPases, crucial regulators of membrane traffic. Here we describe the techniques that we used to characterize the regulated association of Rab18 with the surface of lipid droplets. Rab18 provides an excellent marker to follow the dynamics of lipid droplets in living cells. In addition, the study of Rab18 provides insights into the mechanisms involved in the release of lipids from lipid droplets in adipocytes. In 3T3-L1 adipocytes, stimulation of lipolysis increases the association of Rab18 with lipid droplets, suggesting that recruitment of Rab18 is regulated by the metabolic state of individual lipid droplets. The study of Rab18 and its interacting proteins will provide new insights into the complex regulatory mechanisms involved in lipid storage and release.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
438
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-29
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Characterization of Rab18, a lipid droplet-associated small GTPase.
pubmed:affiliation
Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Queensland, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural