Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-5-26
pubmed:abstractText
Vascular endothelial growth factor (VEGF) is a homodimeric proangiogenic protein that induces endothelial cell migration and proliferation primarily through interactions with its major receptors, VEGFR-1 and VEGFR-2. Inhibitors of one or both of these VEGF-receptor interactions could be beneficial as therapeutics for diseases caused by dysfunctional angiogenesis (e.g., cancer). Others have reported small peptides that bind to the VEGF dimer at surface regions that are recognized by the receptors. Here we report the development of a fluorescence polarization assay based on the binding to VEGF of a derivative of one of these peptides that has been labeled with BODIPY-tetramethylrhodamine (BODIPY(TMR)). This 384-well format assay is tolerant to dimethyl sulfoxide (DMSO, up to 4% [v/v]) and has a Z' factor of 0.76, making it useful for identifying molecules that associate with the receptor-binding surface of the VEGF dimer.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/DK50107, http://linkedlifedata.com/resource/pubmed/grant/EY16995, http://linkedlifedata.com/resource/pubmed/grant/GM56414, http://linkedlifedata.com/resource/pubmed/grant/P30 EY016665-01, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-04, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-05, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-05S1, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-06, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-06S1, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-07, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-07S1, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-08, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-09, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-10A1, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-11, http://linkedlifedata.com/resource/pubmed/grant/R01 DK050107-12, http://linkedlifedata.com/resource/pubmed/grant/R01 EY016695-01A2, http://linkedlifedata.com/resource/pubmed/grant/R01 EY016695-02, http://linkedlifedata.com/resource/pubmed/grant/R01 EY016695-03, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-03, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-04, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-05, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-06, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-07, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-08, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-09, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-10, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-11, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-11S1, http://linkedlifedata.com/resource/pubmed/grant/R01 GM056414-12A1, http://linkedlifedata.com/resource/pubmed/grant/T32 GM008505
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-10471394, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-10543948, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-10878003, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-11001067, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-11001068, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-11080688, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-11080691, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-11730488, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-11866530, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-12006109, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-12137781, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-12778163, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-12778165, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-12854996, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-13678960, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-15060526, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-15105050, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-15136787, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-15246006, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-15464951, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-15610000, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-15645606, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16117535, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16186493, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16301830, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16301831, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16355214, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16484997, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16492159, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16518379, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-16951216, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-17078173, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-17401149, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-17482136, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-17893672, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-18163003, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-2610349, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-8670191, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-8890165, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-9034784, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-9099677, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-9207067, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-9351807, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-9393862, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-9556609, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-9813036, http://linkedlifedata.com/resource/pubmed/commentcorrection/18413228-9922141
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1096-0309
pubmed:author
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