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rdf:type |
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lifeskim:mentions |
umls-concept:C0005456,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0024660,
umls-concept:C0162326,
umls-concept:C0185117,
umls-concept:C1138842,
umls-concept:C1334043,
umls-concept:C1546857,
umls-concept:C2697616,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
1992-11-18
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pubmed:abstractText |
Tissue-specific expression of the maize anthocyanin Bronze-1 (Bz1) gene is controlled by the products of several regulatory genes. These include C1 or Pl and R or B that share homology to the myb proto-oncogenes and myc-like genes, respectively. Bz1 expression in embryo tissues is dependent on C1 and an R-sc allele of R. Transient expression from mutated and deleted versions of the Bz1 promoter fused to a luciferase reporter gene was measured in C1, Rscm2 embryos after gene transfer by microprojectiles. This analysis revealed that the sequences between -76 base pairs (bp) and -45 bp and a 9-bp AT-rich block between -88 bp and -80 bp were critical for Bz1 expression. The -76 bp to -45 bp region includes two short sequences that are homologous to the consensus binding sites of the myb- and myc-like proteins. Site-specific mutations of these "myb" and "myc" sequences reduced Bz1 expression to 10% and 1% of normal, respectively. Additionally, a trimer of a 38-bp oligonucleotide containing these myb and myc sites increased the expression of a cauliflower mosaic virus 35S minimal promoter by 26-fold. This enhancement was dependent on both C1 and R. Because the sites critical for Bz1 expression are homologous to the myb and myc consensus binding sequences and the C1 and R proteins share homology with the myb and myc products, respectively, we propose that C1 and R interact with the Bz1 promoter at these sites.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-16453839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-16578811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-17248885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-1995419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2151660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2152106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2155707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2338243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2403925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2482227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2493990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2535537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2674946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2780550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2798145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2902624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-2974122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-3185713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-3304661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-3396861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-3614381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-3909145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-6241469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-6296253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840914-880210
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:geneSymbol |
Bz1,
C2,
myc
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
317-25
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:1840914-Base Sequence,
pubmed-meshheading:1840914-Binding Sites,
pubmed-meshheading:1840914-Gene Expression Regulation,
pubmed-meshheading:1840914-Genes, Plant,
pubmed-meshheading:1840914-Genes, myc,
pubmed-meshheading:1840914-Molecular Sequence Data,
pubmed-meshheading:1840914-Oncogenes,
pubmed-meshheading:1840914-Organ Specificity,
pubmed-meshheading:1840914-Plant Proteins,
pubmed-meshheading:1840914-Promoter Regions, Genetic,
pubmed-meshheading:1840914-Recombinant Fusion Proteins,
pubmed-meshheading:1840914-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1840914-Species Specificity,
pubmed-meshheading:1840914-Trans-Activators,
pubmed-meshheading:1840914-Zea mays
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pubmed:year |
1991
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pubmed:articleTitle |
C1- and R-dependent expression of the maize Bz1 gene requires sequences with homology to mammalian myb and myc binding sites.
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pubmed:affiliation |
Plant Gene Expression Center, United States Department of Agriculture/University of California, Berkeley, Albany 94710.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
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