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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2008-6-9
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pubmed:abstractText |
A low expression of angiotensinogen in the heart has been construed as indicating a circulating uptake mechanism to explain the local effects of angiotensin II on tissues. The recent identification of angiotensin-(1-12) in an array of rat organs suggests this propeptide may be an alternate substrate for local angiotensin production. To test this hypothesis, tissues from 11-wk-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats (n = 14) were stained with purified antibodies directed to the COOH terminus of angiotensin-(1-12). Robust angiotensin-(1-12) staining was predominantly found in ventricular myocytes with less staining found in the medial layer of intracoronary arteries and vascular endothelium. In addition, angiotensin-(1-12) immunoreactivity was present in the proximal, distal, and collecting renal tubules within the deep cortical and outer medullary zones in both strains. Preadsorption of the antibody with angiotensin-(1-12) abolished staining in both tissues. Corresponding tissue measurements by radioimmunoassay showed 47% higher levels of angiotensin-(1-12) in the heart of SHR compared with WKY rats (P < 0.05). In contrast, renal angiotensin-(1-12) levels were 16.5% lower in SHR compared with the WKY rats (P < 0.05). This study shows for first time the localization of angiotensin-(1-12) in both cardiac myocytes and renal tubular components of WKY and SHR. In addition, we show that increased cardiac angiotensin-(1-12) concentrations in SHR is associated with a small, but statistically significant, reduction in renal angiotensin-(1-12) levels.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-10506489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-10617578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-10690302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-11181790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-11300225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-11357942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-12642013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-12676161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-1323069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-14517166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-15005274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-15748537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-16107577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-16148611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-16816138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-16825422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-17045572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-17397545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-17526648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-17878513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-2204498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-2958609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-3533999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-7768569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-8021006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-8137955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-9022280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-9336385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18408132-9892162
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0363-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
294
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H2614-8
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:18408132-Angiotensinogen,
pubmed-meshheading:18408132-Animals,
pubmed-meshheading:18408132-Antibody Specificity,
pubmed-meshheading:18408132-Disease Models, Animal,
pubmed-meshheading:18408132-Hypertension,
pubmed-meshheading:18408132-Immunohistochemistry,
pubmed-meshheading:18408132-Kidney Tubules,
pubmed-meshheading:18408132-Male,
pubmed-meshheading:18408132-Myocardium,
pubmed-meshheading:18408132-Myocytes, Cardiac,
pubmed-meshheading:18408132-Peptide Fragments,
pubmed-meshheading:18408132-Radioimmunoassay,
pubmed-meshheading:18408132-Rats,
pubmed-meshheading:18408132-Rats, Inbred SHR,
pubmed-meshheading:18408132-Rats, Inbred WKY
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pubmed:year |
2008
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pubmed:articleTitle |
Localization of the novel angiotensin peptide, angiotensin-(1-12), in heart and kidney of hypertensive and normotensive rats.
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pubmed:affiliation |
Hypertension and Vascular Research Center, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. jjessup@wfubmc.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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