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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2008-5-5
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pubmed:abstractText |
Bovine adrenal zona fasciculata (AZF) cells express bTREK-1 K(+) channels that set the resting membrane potential. Inhibition of these channels by adrenocorticotropic hormone (ACTH) is coupled to membrane depolarization and cortisol secretion. Curcumin, a phytochemical with medicinal properties extracted from the spice turmeric, was found to modulate both bTREK-1 K(+) currents and cortisol secretion from AZF cells. In whole-cell patch clamp experiments, curcumin inhibited bTREK-1 with an IC(50) of 0.93muM by a mechanism that was voltage-independent. bTREK-1 inhibition by curcumin occurred through interaction with an external binding site and was independent of ATP hydrolysis. Curcumin produced a concentration-dependent increase in cortisol secretion that persisted for up to 24h. At a maximally effective concentration of 50muM, curcumin increased secretion as much as 10-fold. These results demonstrate that curcumin potently inhibits bTREK-1 K(+) channels and stimulates cortisol secretion from bovine AZF cells. The inhibition of bTREK-1 by curcumin may be linked to cortisol secretion through membrane depolarization. Since TREK-1 is widely expressed in a variety of cells, it is likely that some of the biological actions of curcumin, including its therapeutic effects, may be mediated through inhibition of these K(+) channels.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-11172753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-11301200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-11319556,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-12368289,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-15342638,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-15583024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-15994319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-1621949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-16647042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-16906152,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-17022948,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-17622574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-17803897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-17803898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-17942093,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-3014507,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18406348-9382896
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1090-2104
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
13
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pubmed:volume |
370
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-8
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:18406348-Adrenal Glands,
pubmed-meshheading:18406348-Animals,
pubmed-meshheading:18406348-Cattle,
pubmed-meshheading:18406348-Cells, Cultured,
pubmed-meshheading:18406348-Curcumin,
pubmed-meshheading:18406348-Hydrocortisone,
pubmed-meshheading:18406348-Membrane Potentials,
pubmed-meshheading:18406348-Patch-Clamp Techniques,
pubmed-meshheading:18406348-Potassium Channels, Tandem Pore Domain
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pubmed:year |
2008
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pubmed:articleTitle |
Curcumin inhibits bTREK-1 K+ channels and stimulates cortisol secretion from adrenocortical cells.
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pubmed:affiliation |
Department of Neuroscience, The Ohio State University, College of Medicine and Public Health, 5196 Graves Hall, 333 W.10th Avenue, Columbus, OH 43210-1239, USA. enyeart.1@osu.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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