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rdf:type |
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lifeskim:mentions |
umls-concept:C0003451,
umls-concept:C0017968,
umls-concept:C0024267,
umls-concept:C0039195,
umls-concept:C0205171,
umls-concept:C0301625,
umls-concept:C0332281,
umls-concept:C0392747,
umls-concept:C0443172,
umls-concept:C0521026,
umls-concept:C0546816,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1853126,
umls-concept:C1953353,
umls-concept:C2911692
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pubmed:issue |
4
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pubmed:dateCreated |
1991-4-17
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J04331,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M20869,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M27693,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M61066,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M61067,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M61068,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M61069,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M61070,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M61071,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M64449,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M64450,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M64451,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M64452
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pubmed:abstractText |
Isolates of lymphocytic choriomeningitis virus (LCMV) that elicit a cytotoxic T-lymphocyte response (CTL+) have been compared with isolates that suppress the CTL response (CTL-) in an effort to map this phenotype. A single amino acid change in the glycoprotein of the LCMV Armstrong (ARM) strain is consistently associated with the CTL- trait and the ability of the virus to persist (P+). The CTL+ P- parental strain spontaneously gives rise to CTL- P+ variants within lymphoid tissues of mice persistently infected from birth. To map the structural basis of the phenotype, the complete RNA sequence of LCMV ARM 53b (CTL+) was compared with that of its variant ARM clone 13 (CTL-). Differences in 5 of 10,600 nucleotides were found. Three changes are noted in the large L RNA segment, and two are noted in the small S RNA segment. Only two of the changes distinguishing CTL+ from CTL- isolates affect amino acid coding: lysine to glutamine at amino acid 1079 of the polymerase protein, and phenylalanine to leucine at amino acid 260 of the envelope glycoprotein (GP). We also analyzed two additional CTL- variants and four spontaneous CTL+ revertants. All three CTL- variants differ from the original CTL+ parental strain at GP amino acid 260, indicating that this amino acid change is consistently associated with the CTL- phenotype. By contrast the other four mutations in LCMV are not associated with the CTL- phenotype. Sequence analysis of the coding regions of four CTL+ revertants of ARM clone 13 did not reveal back mutations at the GP 260 locus. This finding indicates that the GP 260 mutation is necessary but not sufficient for a CTL- P+ phenotype and that the reversion to CTL+ P- is likely either due to secondary mutations in other regions of the viral genome or to quasispecies within the revertant population that make significant contributions to the phenotype.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2173792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2212940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2296081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2425264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2434993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2448497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2454989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2473018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2508709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2510401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2555175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2645053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2705303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2705310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2784478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2851083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2967012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-2986004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3084281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3086743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3167982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3259346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3261347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3281762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3467330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3500329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3546729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3882188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-3924414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-4060885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-4133807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-4561841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-4601807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-4622912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-4637295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-4709270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-494491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-6091137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-6332167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-6332201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-6355856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-6492264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-6875516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-7041255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-7144891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840619-794412
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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