Source:http://linkedlifedata.com/resource/pubmed/id/18405431
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2008-4-14
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pubmed:abstractText |
The purpose of the present study was to evaluate the effects of serum-starvation, contact-inhibition and roscovitine treatments on cell-cycle synchronization at the G0/G1 stage of ear skin fibroblasts isolated from transgenic cloned cattle. The developmental competence of re-cloned embryos was also examined. Our results showed that the proportion of G0/G1 cells from the serum-starved group at 3, 4 or 5 days was significantly higher compared with 1 or 2 days only (91.5, 91.7 and 93.5% versus 90.1 and 88.8%, respectively, p < 0.05); whilst there was no statistical difference among cells at 3, 4 or 5 days. For roscovitine-treated cells, the proportion of G0/G1 cells at 2, 3, 4 or 5 days was significantly higher than those treated for 1 day only (91.1, 90.1, 89.4 and 91.3% versus 86.51%, respectively, p < 0.05). The proportion of contact-inhibited G0/G1 cells rose significantly with treatment time, but was similar at 3, 4 and 5 days (89.4, 90.4, 91.4, 91.6 and 92.1%, respectively, p < 0.05). The efficiency of obtaining G0/G1 phase cells was lower when roscovitine treatment was employed to synchronize the cell cycle compared with the serum-starvation and contact-inhibition methods (89.7 versus 91.1% and 91.0%, p < 0.05). Moreover, obvious differences were observed in the rate of fused couplets and blastocysts (89.88 +/- 2.70 versus 87.40 +/- 5.13; 44.10 +/- 8.62 versus 58.38 +/- 13.28, respectively, p < 0.05), when nuclear transfer embryos were reconstructed using donors cells that had been serum starved or contact inhibited for 3 days. Our data indicate that 3 day treatment is feasible for harvesting sufficient G0/G1 cells to produce re-cloned transgenic bovine embryos, regardless of whether serum-starvation, contact-inhibition or roscovitine treatments are used as the synchronization methods.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0967-1994
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
111-6
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pubmed:meshHeading |
pubmed-meshheading:18405431-Animals,
pubmed-meshheading:18405431-Animals, Genetically Modified,
pubmed-meshheading:18405431-Cattle,
pubmed-meshheading:18405431-Cell Cycle,
pubmed-meshheading:18405431-Cells, Cultured,
pubmed-meshheading:18405431-Cloning, Organism,
pubmed-meshheading:18405431-Contact Inhibition,
pubmed-meshheading:18405431-Fibroblasts,
pubmed-meshheading:18405431-Nuclear Transfer Techniques,
pubmed-meshheading:18405431-Oocytes,
pubmed-meshheading:18405431-Protein Kinase Inhibitors,
pubmed-meshheading:18405431-Purines,
pubmed-meshheading:18405431-Serum
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pubmed:year |
2008
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pubmed:articleTitle |
Cell-cycle synchronization of fibroblasts derived from transgenic cloned cattle ear skin: effects of serum starvation, roscovitine and contact inhibition.
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pubmed:affiliation |
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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