Linked Life Data

18405323

Source:http://linkedlifedata.com/resource/pubmed/id/18405323

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-4-14
pubmed:abstractText
CD68, the human homologue of macrosialin, is commonly regarded as a selective marker for human monocytes and macrophages. Its expression is thought to be regulated by a macrophage-specific promoter. However, several immunohistochemical studies have indicated that CD68 antibodies also react with other haematopoietic and non-haematopoietic cell types. We investigated the expression of CD68 in various primary cells and carcinoma cell lines using immunohistochemistry, flow cytometry, Western blot analysis and qRT-PCR. Weak but significant immunoreactivity was detected in lymphocytes and several tumour cell lines whereas staining of primary fibroblasts and endothelial cells was comparable to macrophages. The intensity of CD68 staining in individual cell types depended on the antibody clone and the fixation technique. Anti-CD68 mAb KP1 should be used with great caution for frozen tissue sections due to its reactivity with a wide variety of cell types. Also, care should be taken when distinguishing macrophages from fibroblasts/stromal cells in paraffin sections after formalin fixation since both cell types are stained highly positive for CD68. In accordance, mRNA expression of CD68 was not only detected in macrophages and monocytes but also in fibroblasts as well as endothelial cells and tumour cells, although with a varying intensity. Cloning of full length 5'-sequences and determination of transcription start sites shows that macrophages and fibroblasts initiate transcription within the known promoter region; however, from different start sites, indicating alternative promoter architecture in myeloid versus non-myeloid cells. We suggest that CD68 is not a selective macrophage marker but rather a lysosomal protein that is enriched in macrophages.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1365-3083
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
453-63
pubmed:dateRevised
2009-7-28
pubmed:meshHeading
pubmed-meshheading:18405323-Antigens, CD, pubmed-meshheading:18405323-Antigens, Differentiation, Myelomonocytic, pubmed-meshheading:18405323-Base Sequence, pubmed-meshheading:18405323-Biological Markers, pubmed-meshheading:18405323-Blotting, Western, pubmed-meshheading:18405323-Cell Line, pubmed-meshheading:18405323-Dendritic Cells, pubmed-meshheading:18405323-Fibroblasts, pubmed-meshheading:18405323-Flow Cytometry, pubmed-meshheading:18405323-Humans, pubmed-meshheading:18405323-Immunohistochemistry, pubmed-meshheading:18405323-Lymphocytes, pubmed-meshheading:18405323-Molecular Sequence Data, pubmed-meshheading:18405323-Monocytes, pubmed-meshheading:18405323-Promoter Regions, Genetic, pubmed-meshheading:18405323-RNA, Messenger, pubmed-meshheading:18405323-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18405323-Sensitivity and Specificity, pubmed-meshheading:18405323-Specimen Handling, pubmed-meshheading:18405323-Tissue Fixation, pubmed-meshheading:18405323-Transcription Initiation Site
pubmed:year
2008
pubmed:articleTitle
Expression of CD68 in non-myeloid cell types.
pubmed:affiliation
Department of Hematology and Oncology, University of Regensburg, Regensburg, Germany. eva.gottfried@klinik.uni-regensburg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't