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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-5-24
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pubmed:databankReference |
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pubmed:abstractText |
Myn, a novel murine approximately 18 kd basic/helix-loop-helix/"leucine zipper" (B/HLH/LZ) protein, forms a specific DNA-binding complex with the c-Myc oncoprotein through the HLH/LZ motif in both proteins. c-Myc/Myn recognizes a c-Myc-binding site (GACCACGTGGTC) with higher affinity than either protein by itself. CpG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the c-Myc/Myn complex in vivo. In 3T3 fibroblasts, Myn mRNA levels are induced several-fold by serum with delayed early kinetics, suggesting regulation by immediate-early gene products. Coexpression of Myn in a myc/ras rat embryo fibroblast focus formation assay specifically augmented c-myc transforming activity. We suggest that interaction of Myn with c-Myc stabilizes sequence-specific DNA binding in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
395-407
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1840505-Amino Acid Sequence,
pubmed-meshheading:1840505-Animals,
pubmed-meshheading:1840505-Base Sequence,
pubmed-meshheading:1840505-Basic Helix-Loop-Helix Leucine Zipper Transcription Factors,
pubmed-meshheading:1840505-Blood,
pubmed-meshheading:1840505-Cell Transformation, Neoplastic,
pubmed-meshheading:1840505-Cells, Cultured,
pubmed-meshheading:1840505-DNA,
pubmed-meshheading:1840505-DNA-Binding Proteins,
pubmed-meshheading:1840505-Gene Expression Regulation,
pubmed-meshheading:1840505-Genes, myc,
pubmed-meshheading:1840505-Genes, ras,
pubmed-meshheading:1840505-Leucine Zippers,
pubmed-meshheading:1840505-Methylation,
pubmed-meshheading:1840505-Mice,
pubmed-meshheading:1840505-Molecular Sequence Data,
pubmed-meshheading:1840505-Polymerase Chain Reaction,
pubmed-meshheading:1840505-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:1840505-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
Association of Myn, the murine homolog of max, with c-Myc stimulates methylation-sensitive DNA binding and ras cotransformation.
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pubmed:affiliation |
Howard Hughes Medical Institute, Kaplan Cancer Center, New York, New York.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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