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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1992-3-12
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M63688,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M63689,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M63690,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M63691,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S77311,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S77321,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S77323,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S77325,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S79904,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S79905,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X57797
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pubmed:abstractText |
The temperate bacteriophage phiLC3, isolated from Lactococcus lactis subsp. cremoris, has an isometric head and a flexible tail containing 1 major protein and 8 minor proteins. Infection of a permissive L. lactis host strain yields a burst of about 50 phages per infected cell with a latent period of 60 min. A detailed restriction map of the phage chromosome was constructed by using 12 different restriction enzymes. The phage chromosome is a 33-kb linear double-stranded DNA molecule with unique cohesive ends and with a G + C content of 36.5%. Chemical sequencing of the DNA ends revealed 13-base 3' extended complementary single strands with a relatively high percentage of G + C. Pulsed-field gel electrophoretic analysis of DNA from a strain lysogenized with phiLC3 was used to localize the prophage to a 320-kb BamHI restriction endonuclease fragment from the host chromosomal DNA. This result indicates that lysogeny involves integration of the phage into the host chromosome. A spontaneous phiLC3 clear plaque mutant that was unable to give rise to lysogens was isolated.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-16345892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-16346534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-16347374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-16347600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-16350018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-2111118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-2140565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-2167627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-2271226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-2881846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-2999317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-3008648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-3128635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-362149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-3927011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-402110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-4865539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-6271624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-6324674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-6335448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1840480-875026
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3206-11
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1840480-Bacteriophages,
pubmed-meshheading:1840480-Base Composition,
pubmed-meshheading:1840480-Base Sequence,
pubmed-meshheading:1840480-DNA, Single-Stranded,
pubmed-meshheading:1840480-DNA, Viral,
pubmed-meshheading:1840480-Genome, Viral,
pubmed-meshheading:1840480-Lactococcus lactis,
pubmed-meshheading:1840480-Lysogeny,
pubmed-meshheading:1840480-Molecular Sequence Data,
pubmed-meshheading:1840480-Mutation,
pubmed-meshheading:1840480-Viral Plaque Assay,
pubmed-meshheading:1840480-Viral Proteins
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pubmed:year |
1991
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pubmed:articleTitle |
Characterization of phiLC3, a Lactococcus lactis subsp. cremoris temperature bacteriophage with cohesive single-stranded DNA ends.
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pubmed:affiliation |
Laboratory of Microbial Gene Technology, Oslo, Norway.
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pubmed:publicationType |
Journal Article
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