|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2008-6-30
|
|
pubmed:abstractText |
The Na(+)/H(+) exchanger has been the only unequivocally demonstrated H(+)-transport mechanism in the synaptosomal preparation. We had previously suggested that a Cl(-)-H(+) symporter (in its acidifying mode) is involved in cytosolic pH regulation in the synaptosomal preparation. Supporting this suggestion, we now show that: (1) when synaptosomes are transferred from PSS to either gluconate or sulfate solutions, the Fura-2 ratio remains stable instead of increasing as it does in 50 mM K solution. This indicates that these anions do not promote a plasma membrane depolarization. (2) Based in the recovery rate from the cytosolic alkalinization, the anionic selectivity of the Cl(-)-H(+) symporter is NO(3)(-) > Br(-) > Cl(-) >> I(-) = isethionate = sulfate = methanesulfonate = gluconate. (3) PCMB 10 muM inhibits the gluconate-dependent alkalinization by 30 +/- 6%. (4) Neither Niflumic acid, 9AC, Bumetanide nor CCCP inhibits the recovery from the cytosolic alkalinization.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anions,
http://linkedlifedata.com/resource/pubmed/chemical/Antiporters,
http://linkedlifedata.com/resource/pubmed/chemical/Arylsulfonates,
http://linkedlifedata.com/resource/pubmed/chemical/Bumetanide,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonyl Cyanide m-Chlorophenyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Gluconates,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/Niflumic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Potassium Chloride...,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfates,
http://linkedlifedata.com/resource/pubmed/chemical/gluconic acid,
http://linkedlifedata.com/resource/pubmed/chemical/hydrogen-chloride symporter,
http://linkedlifedata.com/resource/pubmed/chemical/pyranine
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1573-6903
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
33
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1574-81
|
|
pubmed:meshHeading |
pubmed-meshheading:18404376-Amino Acid Sequence,
pubmed-meshheading:18404376-Animals,
pubmed-meshheading:18404376-Anions,
pubmed-meshheading:18404376-Antiporters,
pubmed-meshheading:18404376-Arylsulfonates,
pubmed-meshheading:18404376-Brain,
pubmed-meshheading:18404376-Bumetanide,
pubmed-meshheading:18404376-Calcium,
pubmed-meshheading:18404376-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:18404376-Fluorescent Dyes,
pubmed-meshheading:18404376-Gluconates,
pubmed-meshheading:18404376-Hydrogen-Ion Concentration,
pubmed-meshheading:18404376-Ionophores,
pubmed-meshheading:18404376-Niflumic Acid,
pubmed-meshheading:18404376-Potassium,
pubmed-meshheading:18404376-Rats,
pubmed-meshheading:18404376-Sodium Potassium Chloride Symporter Inhibitors,
pubmed-meshheading:18404376-Sulfates,
pubmed-meshheading:18404376-Synaptosomes
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Anionic selectivity sequence of the Cl(-)-H+ symporter in the synaptosomal preparation from rat brain cortex.
|
|
pubmed:affiliation |
Departamento de Fisiología y Farmacología, Facultad de Medicina, Universidad Autónoma de San Luis Potosi, Av. V. Carranza 2405, San Luis Potosi, S.L.P., 78210, Mexico.
|
|
pubmed:publicationType |
Journal Article
|
