Source:http://linkedlifedata.com/resource/pubmed/id/18403636
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2008-4-11
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| pubmed:abstractText |
To further understand the biological significance of amplifications for glioma development and recurrencies, we characterized amplicon frequency and size in low-grade glioma and amplicon stability in vivo in recurring glioblastoma. We developed a 12q13-21 amplicon-specific genomic microarray and a bioinformatics amplification prediction tool to analyze amplicon frequency, size, and maintenance in 40 glioma samples including 16 glioblastoma, 10 anaplastic astrocytoma, 7 astrocytoma WHO grade 2, and 7 pilocytic astrocytoma. Whereas previous studies reported two amplified subregions, we found a more complex situation with many amplified subregions. Analyzing 40 glioma, we found that all analyzed glioblastoma and the majority of pilocytic astrocytoma, grade 2 astrocytoma, and anaplastic astrocytoma showed at least one amplified subregion, indicating a much higher amplification frequency than previously suggested. Amplifications in low-grade glioma were smaller in size and displayed clearly different distribution patterns than amplifications in glioblastoma. One glioblastoma and its recurrencies revealed an amplified subregion of 5 Mb that was stable for 6 years. Expression analysis of the amplified region revealed 10 overexpressed genes (i.e., KUB3, CTDSP2, CDK4, OS-9, DCTN2, RAB3IP, FRS2, GAS41, MDM2, and RAP1B) that were consistently overexpressed in all cases that carried this amplification. Our data indicate that amplifications on 12q13-21 (a) are more frequent than previously thought and present in low-grade tumors and (b) are maintained as extended regions over long periods of time.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1541-7786
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
6
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
576-84
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| pubmed:meshHeading |
pubmed-meshheading:18403636-Adult,
pubmed-meshheading:18403636-Aged,
pubmed-meshheading:18403636-Blotting, Southern,
pubmed-meshheading:18403636-Carbocyanines,
pubmed-meshheading:18403636-Child,
pubmed-meshheading:18403636-Child, Preschool,
pubmed-meshheading:18403636-Chromosomes, Artificial, Bacterial,
pubmed-meshheading:18403636-Chromosomes, Human, Pair 12,
pubmed-meshheading:18403636-Computational Biology,
pubmed-meshheading:18403636-Cosmids,
pubmed-meshheading:18403636-DNA, Neoplasm,
pubmed-meshheading:18403636-Female,
pubmed-meshheading:18403636-Gene Amplification,
pubmed-meshheading:18403636-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18403636-Genes, Neoplasm,
pubmed-meshheading:18403636-Glioma,
pubmed-meshheading:18403636-Humans,
pubmed-meshheading:18403636-In Situ Hybridization, Fluorescence,
pubmed-meshheading:18403636-Male,
pubmed-meshheading:18403636-Middle Aged,
pubmed-meshheading:18403636-Oligonucleotide Array Sequence Analysis
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| pubmed:year |
2008
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| pubmed:articleTitle |
A different view on DNA amplifications indicates frequent, highly complex, and stable amplicons on 12q13-21 in glioma.
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| pubmed:affiliation |
Department of Human Genetics, Saarland University, 66421 Homburg/Saar, Germany. hgufis@uniklinik-saarland.de
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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