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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1992-7-13
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pubmed:abstractText |
Free and total fatty acids in the blood serum of patients with hyperlipoproteinemia have been analysed as their methyl esters by capillary gas chromatography using an FFAP column. In one-step reactions the free fatty acids in serum react with methanol-acetyl chloride (50:1, v/v) at 25 degrees C, the total fatty acids (free plus esterified) are transesterified with methanol-toluene-acetyl chloride (8:2:1, v/v) at 100 degrees C. The quantification of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is based on an internal standard (13,16,19-docosatrienoic acid) and on calibration standards. Under normal diet the concentrations of EPA and DHA are as follows (mean +/- S.D., n = 27): free EPA, 0.2 +/- 0.1 mg/dl; free DHA, 0.6 +/- 0.2 mg/dl; total EPA, 3.6 +/- 2.1 mg/dl; total DHA 11.4 +/- 3.1 mg/dl. Under a fish oil intake of 9 g per day, free and total EPA concentrations rise by ca. five- to six-fold, and free and total DHA concentrations by ca. two-fold.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Docosahexaenoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Eicosapentaenoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Fish Oils,
http://linkedlifedata.com/resource/pubmed/chemical/Placebos
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
572
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1840144-Chromatography, Gas,
pubmed-meshheading:1840144-Docosahexaenoic Acids,
pubmed-meshheading:1840144-Eicosapentaenoic Acid,
pubmed-meshheading:1840144-Esters,
pubmed-meshheading:1840144-Fish Oils,
pubmed-meshheading:1840144-Humans,
pubmed-meshheading:1840144-Hyperlipoproteinemias,
pubmed-meshheading:1840144-Placebos
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pubmed:year |
1991
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pubmed:articleTitle |
Analysis of polyunsaturated fatty acids in blood serum after fish oil administration.
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pubmed:affiliation |
Medizinische Universitätsklinik, Tübingen, Germany.
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pubmed:publicationType |
Journal Article
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