|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2008-4-30
|
|
pubmed:abstractText |
Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured by semiquantitative reverse transcriptase PCR and its protein secretion was assayed by gelatin zymography. Prevotella intermedia ATCC 25611 supernatant time and dose-dependently induced MMP-9 expression. In contrast, Porphyromanas gingivalis ATCC 33277 supernatants, Escherichia coli lipopolysacchride and IL-1beta exhibited no stimulatory effects on MMP-9 production in hPDL cells. Mitogen-activated protein kinases [MAPK, including extracellular signal-related kinases (ERK), c-jun N-terminal kinases (JNK) and p38] inhibitors exerted no effect on the P. intermedia-induced MMP-9 production, indicating that P. intermedia induced MMP-9 production through an MAPK-independent pathway. Our results demonstrated that P. intermedia may contribute to periodontal tissue destruction during chronic periodontitis by inducing MMP-9 production in hPDL cells.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anthracenes,
http://linkedlifedata.com/resource/pubmed/chemical/Butadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/SB 239063,
http://linkedlifedata.com/resource/pubmed/chemical/U 0126,
http://linkedlifedata.com/resource/pubmed/chemical/anthra(1,9-cd)pyrazol-6(2H)-one,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0378-1097
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
283
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
47-53
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:18400000-Adolescent,
pubmed-meshheading:18400000-Anthracenes,
pubmed-meshheading:18400000-Bacteroidaceae Infections,
pubmed-meshheading:18400000-Butadienes,
pubmed-meshheading:18400000-Cells, Cultured,
pubmed-meshheading:18400000-Child,
pubmed-meshheading:18400000-Culture Media, Conditioned,
pubmed-meshheading:18400000-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:18400000-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:18400000-Humans,
pubmed-meshheading:18400000-Imidazoles,
pubmed-meshheading:18400000-Interleukin-1beta,
pubmed-meshheading:18400000-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:18400000-Lipopolysaccharides,
pubmed-meshheading:18400000-Matrix Metalloproteinase 9,
pubmed-meshheading:18400000-Nitriles,
pubmed-meshheading:18400000-Periodontal Ligament,
pubmed-meshheading:18400000-Porphyromonas gingivalis,
pubmed-meshheading:18400000-Prevotella intermedia,
pubmed-meshheading:18400000-Protein Kinase Inhibitors,
pubmed-meshheading:18400000-Pyrimidines,
pubmed-meshheading:18400000-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18400000-Time Factors,
pubmed-meshheading:18400000-p38 Mitogen-Activated Protein Kinases
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Prevotella intermedia induces matrix metalloproteinase-9 expression in human periodontal ligament cells.
|
|
pubmed:affiliation |
Department of Oral Biology, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
