Source:http://linkedlifedata.com/resource/pubmed/id/18392977
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2008-4-8
|
| pubmed:abstractText |
A method is described for generating conditional lethal mutations in essential genes in Escherichia coli. In this procedure, amber stop codons are introduced as "tagalong" mutations in the flanking DNA of a downstream antibiotic-resistance marker by lambda Red recombination. The marker is removed by expression of I-SceI homing endonuclease, leaving a markerless mutation. The mutants then depend upon expression of a suppressor transfer RNA (tRNA) for survival, which is expressed under control of the arabinose promoter on a high-copy-number plasmid.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1064-3745
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
416
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
323-34
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| pubmed:meshHeading |
pubmed-meshheading:18392977-Anti-Bacterial Agents,
pubmed-meshheading:18392977-Escherichia coli,
pubmed-meshheading:18392977-Gene Expression Regulation, Bacterial,
pubmed-meshheading:18392977-Genes, Bacterial,
pubmed-meshheading:18392977-Genes, Essential,
pubmed-meshheading:18392977-Genes, Suppressor,
pubmed-meshheading:18392977-Mutation,
pubmed-meshheading:18392977-RNA, Bacterial,
pubmed-meshheading:18392977-RNA, Transfer, Amino Acyl
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| pubmed:year |
2008
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| pubmed:articleTitle |
Introduction of conditional lethal amber mutations in Escherichia coli.
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| pubmed:affiliation |
Mascoma Corporation, Lebanon, NH, USA.
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| pubmed:publicationType |
Journal Article
|