Linked Life Data

18391762

Source:http://linkedlifedata.com/resource/pubmed/id/18391762

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-4-24
pubmed:abstractText
Adoptive cell transfer may be a successful strategy in anticancer therapy and its therapeutic efficiency depends on the access of transferred cells to the tumor site and their persistence in vivo. Nevertheless, the migration properties of autologous in vitro-activated T cells in primates are largely unknown. Here, we established the long-term tracking of T-cell migration into various compartments of rhesus macaques as a preclinical model for the evaluation of T-cell-based immunotherapy. Peripheral blood mononuclear cells from 3 to 4 rhesus macaques were activated with anti-CD3/anti-CD28 or not, labeled with carboxyfluorescein diacetat succinimidyl ester, and reinjected intravenously into the donor animals. Blood samples, lymph node biopsies, and mucosal biopsies (duodenum, rectum) were collected at various time points and analyzed by flow cytometry for the presence of the reinjected T cells. We demonstrate that nonspecific in vitro activation changes the in vivo migratory behavior of T cells and provokes a preferential migration of CD8 T cells to the rectum. Nonspecifically activated transferred CD4 T cells were found in much lower frequencies at this site and also in other compartments. Thus, our results indicate an imbalanced distribution of autologous CD8 and CD4 T cells in various compartments that is more apparent when T cells are activated before the transfer. The migratory behavior of in vitro-expanded, autologously transferred T cells can, therefore, influence the clinical outcome of adoptive cell transfer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1524-9557
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
334-44
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Migration patterns of nonspecifically activated versus nonactivated nonhuman primate T lymphocytes: preferential homing of activated autologous CD8+ T cells in the rectal mucosa.
pubmed:affiliation
Medical Clinic I, Gastroenterology, Infectious Diseases and Rheumatology, Charité-Campus Benjamin Franklin, Berlin, Germany. kristina.allers@charite.de
pubmed:publicationType
Journal Article