Linked Life Data

18386025

Source:http://linkedlifedata.com/resource/pubmed/id/18386025

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-7-30
pubmed:abstractText
In order to isolate and culture the sweat gland epithelial cells in vitro and to study the effects of acetylcholine (ACh) on intracellular calcium concentration ([Ca(2+)](i)) of cultured sweat gland epithelial cells, the following methods were used. First, repeated shearing and neutral red staining made the sweat glands pop out from subcutaneous tissues. Then, transferpettor was used to pick up the glands, which were cultured in Epilife after type II collagenase digestion. The molecular characterization of primary cultured sweat gland epithelial cells was shown by immunocytochemistry. The [Ca(2+)](i) was examined by confocal laser scanning microscopy (CLSM) with the Ca(2+)-sensitive dye Fura 3-AM, when ACh was added to the primary cells and the first passage cells. In the results, the established method yielded comparatively more sweat glands, and the primary and first passage epithelial cells developed well in Epilife. The primary epithelial cells were positive to anti-EMA, anti-CK and anti-CK7. After the ACh was added, when the medium with high calcium (2 mmol/L) was applied, the calcium channel of both primary and first passage cells opened and significant [Ca(2+)](I )increase was observed; when the medium with no calcium was applied, no significant [Ca(2+)](i )increase was observed. So, it is a good method to isolate sweat glands by repeated shearing and transferpettor picking, and the culture mediums of keratinocytes, like Epilife, can be used to culture the sweat glands epithelial cells. In both the cultured primary and first passage cells, when stimulated by ACh, the calcium channel opened, which induced an increase in [Ca(2+)](i), similar to the cells in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1432-069X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
300
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
335-41
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Effects of acetylcholine chloride on intracellular calcium concentration of cultured sweat gland epithelial cells.
pubmed:affiliation
Department of Dermatology, Daping Hospital, The Third Military Medical University, Chongqing, China. leixia1979@sina.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't