Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2008-4-9
pubmed:abstractText
Recent evidence suggests a role for nanobacteria in a growing number of human diseases, including renal stone formation, cardiovascular diseases, and cancer. This large body of research studies promotes the view that nanobacteria are not only alive but that they are associated with disease pathogenesis. However, it is still unclear whether they represent novel life forms, overlooked nanometer-size bacteria, or some other primitive self-replicating microorganisms. Here, we report that CaCO(3) precipitates prepared in vitro are remarkably similar to purported nanobacteria in terms of their uniformly sized, membrane-delineated vesicular shapes, with cellular division-like formations and aggregations in the form of colonies. The gradual appearance of nanobacteria-like particles in incubated human serum as well as the changes seen with their size and shape can be influenced and explained by introducing varying levels of CO(2) and NaHCO(3) as well as other conditions known to influence the precipitation of CaCO(3). Western blotting reveals that the monoclonal antibodies, claimed to be specific for nanobacteria, react in fact with serum albumin. Furthermore, nanobacteria-like particles obtained from human blood are able to withstand high doses of gamma-irradiation up to 30 kGy, and no bacterial DNA is found by performing broad-range PCR amplifications. Collectively, our results provide a more plausible abiotic explanation for the unusual properties of purported nanobacteria.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-10844606, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-11027350, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-12454193, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-12517874, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-12602586, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-12938934, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-14615534, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-15569055, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-15643213, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-15673296, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-16364440, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-16582542, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-16593827, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-16674119, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-16706890, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-16865581, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-8688069, http://linkedlifedata.com/resource/pubmed/commentcorrection/18385376-9653177
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
8
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5549-54
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Purported nanobacteria in human blood as calcium carbonate nanoparticles.
pubmed:affiliation
Department of Biochemistry and Cellular Molecular Biology, Chang Gung University, 259 Wen-Hua First Road, Kwei-Shan, Tao-Yuan 333, Taiwan, Republic of China. jan.martel@usherbrooke.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't