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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-3-23
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pubmed:abstractText |
1. Particulate and cytosolic protein kinase C (PKC) activity was measured in rat aortae with and without endothelium, following exposure to endothelin-1 (10(-8) M) for various time intervals. 2. Endothelin-1 induced two peaks of particulate PKC activity, occurring at 30 s and 10 min exposure times in both endothelium-intact and endothelium-denuded preparations. Cytosolic PKC activity fell below baseline at all incubation times studied. 3. In endothelium-denuded preparations, elevation of guanosine 3':5'-cyclic monophosphate (cyclic GMP) levels with sodium nitroprusside (10(-6) M) or atrial natriuretic peptide (10(-6) M) and, in endothelium-intact preparations with the calcium ionophore A23187 (10(-6) M), inhibited the activation of particulate PKC activity seen after incubation with endothelin-1 for 30 s. The inhibitory effect of A23187 was prevented by prior incubation of the endothelium-intact vessels with the nitric oxide synthetase inhibitor, L-NG-nitro arginine (5 x 10(-5) M). 4. These results indicate that EDRF acting via cyclic GMP can inhibit the activation of PKC induced by endothelin-1 in rat aorta.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-1646060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-1972016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2015428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2142097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2153123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2166789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2205202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2328404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2403981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2451132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2525783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2541708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2559197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2597139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2656676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2679147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2733552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2843639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-2845214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-3013461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-3040504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-3042836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-3084474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-3144974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-3160705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-3495737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-6095092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-6232463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1838492-6269550
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1838492-Animals,
pubmed-meshheading:1838492-Aorta, Thoracic,
pubmed-meshheading:1838492-Arginine,
pubmed-meshheading:1838492-Atrial Natriuretic Factor,
pubmed-meshheading:1838492-Calcimycin,
pubmed-meshheading:1838492-Cytosol,
pubmed-meshheading:1838492-Endothelins,
pubmed-meshheading:1838492-Endothelium, Vascular,
pubmed-meshheading:1838492-Enzyme Activation,
pubmed-meshheading:1838492-Male,
pubmed-meshheading:1838492-Muscle, Smooth, Vascular,
pubmed-meshheading:1838492-Nitric Oxide,
pubmed-meshheading:1838492-Nitroarginine,
pubmed-meshheading:1838492-Nitroprusside,
pubmed-meshheading:1838492-Protein Kinase C,
pubmed-meshheading:1838492-Rats,
pubmed-meshheading:1838492-Rats, Inbred Strains
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pubmed:year |
1991
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pubmed:articleTitle |
Endothelium-derived relaxing factor inhibits the endothelin-1-induced increase in protein kinase C activity in rat aorta.
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pubmed:affiliation |
Department of Pharmacology & Therapeutics, University of Wales College of Medicine, Heath Park, Cardiff.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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