Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-4-3
pubmed:abstractText
Digital autoradiography (DAR) is a powerful method to determine quantitatively the "small-scale" (i.e., submillimeter) distribution of a radiotracer within a tissue section. However, the limited spatial resolution of the DAR image, due to blurring by the point spread function (PSF), can result in a poor correlation with tissue histology and immunohistochemistry. The authors attempt to overcome this limitation by recovering the radiotracer distribution by image deconvolution using the Richardson-Lucy algorithm and a measured PSF obtained from a small radioactive source on hydrophobic microscope slide. Simulation studies have shown that the deconvolution algorithm reliably recovers the pixel values corresponding to the radioactivity distributions. As an example, the proposed image restoration approach has been tested with DAR images of different radiolabeled markers on tumor sections obtained from clinical and preclinical animal model studies. Digital autoradiograms following deconvolution show improved sharpness and contrast relative to the unprocessed autoradiograms.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-10816266, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-11079503, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-11156414, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-14523586, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-18238264, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-3185726, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-3681517, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-3683303, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-6878707, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-8205788, http://linkedlifedata.com/resource/pubmed/commentcorrection/18383673-8496726
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0094-2405
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
522-30
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Image deconvolution in digital autoradiography: a preliminary study.
pubmed:affiliation
Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA.
pubmed:publicationType
Journal Article, Evaluation Studies, Research Support, N.I.H., Extramural