Linked Life Data

18378427

Source:http://linkedlifedata.com/resource/pubmed/id/18378427

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-7-9
pubmed:abstractText
The objective of this study was to evaluate the role of collagen membrane and Bio-Oss coverage in healing of an onlay graft to the mandible. Twelve adult sheep each received an onlay bone graft (experiment 1), bone graft+Bio-Gide (experiment 2), and bone graft+Bio-Oss/Bio-Gide (experiment 3) on the lateral surface of the mandible. The animals were euthanized at 4, 8, 12 or 16 weeks after surgery, and findings were analysed by routine microscopy and immunohistochemistry for proliferation (Ki67) and apoptotic (Caspase-3) markers. Grafts were fully incorporated in all specimens. Pronounced resorption was observed in experiment 1. Minimal loss of graft volume was seen in experiment 2 specimens without membrane displacement. A remarkable increase in the augmented region of the mandible was observed in experiment 3. A high number of osteoclasts were expressed within the grafts during the early healing period, and thereafter declined markedly. Osteoblasts within the grafts expressed a moderate level of Ki67 at 8 weeks, which thereafter declined markedly. The strongest expression of Caspase-3 on the bone surface was observed after 16 weeks. In conclusion, the effect of collagen membrane coverage on bone graft volume maintenance was dependent on membrane stability during healing. An autogenous bone graft covered with Bio-Oss particles resulted in a remarkable increase in augmented lateral surface of the mandible. The late stage of bone graft healing was associated with a high apoptotic induction pathway of osteoblasts lining the surfaces of the new bone, demonstrated by strong positive Caspase-3 immunoreactivity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0901-5027
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
651-9
pubmed:meshHeading
pubmed-meshheading:18378427-Animals, pubmed-meshheading:18378427-Apoptosis, pubmed-meshheading:18378427-Biocompatible Materials, pubmed-meshheading:18378427-Biological Markers, pubmed-meshheading:18378427-Bone Matrix, pubmed-meshheading:18378427-Bone Resorption, pubmed-meshheading:18378427-Bone Substitutes, pubmed-meshheading:18378427-Bone Transplantation, pubmed-meshheading:18378427-Caspase 3, pubmed-meshheading:18378427-Cattle, pubmed-meshheading:18378427-Cell Proliferation, pubmed-meshheading:18378427-Collagen, pubmed-meshheading:18378427-Female, pubmed-meshheading:18378427-Graft Survival, pubmed-meshheading:18378427-Ki-67 Antigen, pubmed-meshheading:18378427-Mandible, pubmed-meshheading:18378427-Membranes, Artificial, pubmed-meshheading:18378427-Minerals, pubmed-meshheading:18378427-Osteoblasts, pubmed-meshheading:18378427-Osteoclasts, pubmed-meshheading:18378427-Random Allocation, pubmed-meshheading:18378427-Sheep, pubmed-meshheading:18378427-Time Factors, pubmed-meshheading:18378427-Wound Healing
pubmed:year
2008
pubmed:articleTitle
Healing of onlay mandibular bone grafts covered with collagen membrane or bovine bone substitutes: a microscopical and immunohistochemical study in the sheep.
pubmed:affiliation
Department of Craniomaxillofacial and Plastic Surgery, University of Cologne, Germany. lanreadeyemo@yahoo.com
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't