Source:http://linkedlifedata.com/resource/pubmed/id/18374088
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2008-3-31
|
pubmed:abstractText |
Replacement of beta-cell mass offers an alternative to standard insulin treatment for diabetes and may overcome the long-term side effects associated with current therapies. Pancreatic stem/progenitor cells could become a useful target for beta-cell replacement therapy in diabetic patients. We have established a method for isolating mouse pancreatic stem cells. In this study, pancreatic stem cells were isolated from 8-week-old mice. After purification on a density gradient, the density range of 1.062-1.11 contained pancreatic stem cells. The islets from the layers were deleted by dithizone staining and hand-picking under a dissecting microscope. The remnant cells were then cultured, inoculated into 96-well plates, and cloned by limiting dilution. One of the wells contained cells, named HN#5 cells, which expressed ductal cell markers, such as cytokeratin-19. HN#5 cells differentiated into insulin-producing cells and albumin-producing cells by induction medium. The isolation technique described here may be useful for identification and isolation of human pancreatic stem/progenitor cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0041-1345
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
40
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
422-3
|
pubmed:meshHeading |
pubmed-meshheading:18374088-Animals,
pubmed-meshheading:18374088-Cell Culture Techniques,
pubmed-meshheading:18374088-Cell Line,
pubmed-meshheading:18374088-Cell Separation,
pubmed-meshheading:18374088-Centrifugation, Density Gradient,
pubmed-meshheading:18374088-Clone Cells,
pubmed-meshheading:18374088-Insulin-Secreting Cells,
pubmed-meshheading:18374088-Mice,
pubmed-meshheading:18374088-Pancreas,
pubmed-meshheading:18374088-Pancreatic Ducts,
pubmed-meshheading:18374088-Stem Cells
|
pubmed:year |
2008
|
pubmed:articleTitle |
Method for isolation of mouse pancreatic stem cells.
|
pubmed:affiliation |
Baylor Institute for Immunology Research/Baylor All Saints Medical Center, Baylor Research Institute, Fort Worth, Texas 75204, USA. hirofumn@baylorhealth.edu
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|