Linked Life Data

18371630

Source:http://linkedlifedata.com/resource/pubmed/id/18371630

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-3-28
pubmed:abstractText
A new fast and reproducible approach is described for the application of the enzymatic probe sonication (EPS) methodology [J.L. Capelo, P. Ximénez-Embún, Y. Madrid-Albarrán, C. Cámara, Anal. Chem. 76 (2004) 233-237] for total selenium determination by electrothermal atomic absorption spectrometry, ET-AAS. Ni(NO3)2 and Pd(NO3)2 were studied as matrix modifiers in conjunction with H2O2, being best results obtained with Pd(NO3)2 plus H2O2. The presence of H2O2 as matrix modifier increases up to 66% the time-life of the graphite tubes, by avoiding the building-up of carbonaceous residues. BCR-414 plankton and ERM-CE 278 mussel tissue reference materials were used for proof-of-the-methodology. Different enzymes, protease XIV, substilisin and trypsin were studied. The use of fresh enzyme was found critical. Good Se recoveries were obtained for oyster tissue, 111%; BCR-414 plankton, 106% and ERM-CE 278 mussel tissue, 93%, when protease XIV was used. Data regarding microwave digestion versus EPS methodology is also presented and discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1873-3573
pubmed:author
pubmed:issnType
Electronic
pubmed:day
30
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
198-205
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Enzymatic probe sonication as a tool for solid-liquid extraction for total selenium determination by electrothermal-atomic absorption spectrometry.
pubmed:affiliation
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte da Caparica, Portugal.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't