Linked Life Data

18370283

Source:http://linkedlifedata.com/resource/pubmed/id/18370283

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-3-28
pubmed:abstractText
Since their conception in the late 1990s, microarray techniques have become a tool of choice for monitoring pangenomic gene expression. Although there are a large number of variations on the basic methodology the general approach remains standard and involves the comparison of a "test" RNA with a "control" RNA; in this case "healthy" and "virus-infected" plants. The protocol itself can be broken down into five main parts: RNA extraction, cDNA synthesis, hybridization, array scanning, and data analysis. The method presented is optimized for use with arrays based on glass slides spotted with cDNA, in this case 15,264 cDNAs from Solanum tuberosum. The labeling technique presented involves two steps: hybridization of cDNA produced using oligo-dT linker primers to the array and hybridization with a DNA dendrimer reagent comprising sequence complementary to the linker sequence bound to a fluorescent dye. We also present the use of the R environment for data analysis, generating statistical support for differential gene expression observed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
451
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
583-613
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Expression microarrays in plant-virus interaction.
pubmed:affiliation
Department of Biotechnology and Systems Biology, National Institute of Biology, Vecna pot 111, 1000, Ljubljana, Slovenia.
pubmed:publicationType
Journal Article