Linked Life Data

18370008

Source:http://linkedlifedata.com/resource/pubmed/id/18370008

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-3-28
pubmed:abstractText
Sub-cellular proteomics has proven to be a powerful approach to link the information contained in sequenced genomes from eukaryotic cells to the functional knowledge provided by studies of cell compartments. Chloroplasts are plant-specific organelles and are the site of photosynthesis and also of many other essential metabolic pathways, like syntheses of amino acids, vitamins, and pigments. They contain several sub-organellar compartments: the envelope (the two-membrane system surrounding the organelle), the stroma (the internal soluble phase), and the thylakoid membranes (the internal membrane system). There is a link between these compartments and the functions of their constitutive proteins. One way to bring into view the sub-proteomes of the chloroplast is to develop proteomic analyses based (1) on the use of highly purified sub-fractions of the chloroplast and (2) on mass spectrometry (MS)-based analyses for protein identification. To illustrate such strategies, this chapter describes the methods for purification of chloroplasts from Arabidopsis leaves and for the specific recovery of highly pure sub-organellar fractions of envelope, stroma, and thylakoids. Subsequently, methods are described to analyze by MS the proteins recovered from these fractions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
432
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19-36
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Purification and proteomic analysis of chloroplasts and their sub-organellar compartments.
pubmed:affiliation
Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France.
pubmed:publicationType
Journal Article