Linked Life Data

18369778

Source:http://linkedlifedata.com/resource/pubmed/id/18369778

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-3-28
pubmed:abstractText
The use of siRNA in vivo as well as in animal models has become more widespread in recent years, leading to further questions as to the best mode of delivery that will achieve optimal knockdown. While the exact mechanism of siRNA uptake at a cellular level has yet to be fully elucidated, various delivery techniques are being researched, including the use of viral vectors of shRNA, liposome encapsulations, and hydrodynamic delivery of naked siRNA. We describe the use of hydrodynamic administration as a technique to deliver, in vivo, naked siRNA constructs into experimental animals as a method of transient gene knockdown. This method may prove useful in situations where knockout animals do not exist, or to determine the effect of gene knockdown at specific time points during an experiment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
442
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-73
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Hydrodynamic delivery of siRNA in a mouse model of sepsis.
pubmed:affiliation
Department of Surgery, Rhode Island Hospital/Brown University School of Medicine, Division of Surgical Research, Providence, RI, USA.
pubmed:publicationType
Journal Article