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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2008-7-11
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pubmed:abstractText |
Serotonin antagonists show impressive analgesic efficacy in rheumatoid arthritis, osteoarthritis (OA) or fibromyalgia; however, this effect is not well understood. We examined the mechanism of serotonin-induced inflammation and its antagonists in OA. Serotonin receptor subtypes and COX-2 were analysed by RT-PCR from synovial tissue. Serum-free cultures were stimulated with 10 muM serotonin and/or the antagonists ketanserin (5-HT(2A)), tropisetron (5-HT(3)) and parecoxib (COX-2). Prostaglandin E(2) (PGE(2)), tumour necrosis factor alpha (TNF-alpha), interleukin 1beta (IL-1beta) and leukotriene B4 (LTB4) were measured by an immunoassay in the supernatants. RT-PCR results showed mRNA for 5-HT(2A) and 5-HT(3) receptors, and COX-2. PGE(2) in the supernatants increased by 261.2% +/- 56.7 (mean +/- SEM; P = 0.007) in response to serotonin. TNF-alpha, IL-1beta and LTB4 levels did not change. Ketanserin, tropisetron and parecoxib suppressed PGE(2). The serotonin-induced PGE(2) overexpression appeared thus to be mediated by 5-HT(2A) and 5-HT(3) receptors. This activation might involve COX-2. The findings may explain the potent benefit of 5-HT(3) antagonists.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta,
http://linkedlifedata.com/resource/pubmed/chemical/Isoxazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Ketanserin,
http://linkedlifedata.com/resource/pubmed/chemical/Leukotriene B4,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Serotonin, 5-HT2A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin, 5-HT3,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/parecoxib,
http://linkedlifedata.com/resource/pubmed/chemical/tropisetron
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0172-8172
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1017-22
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pubmed:meshHeading |
pubmed-meshheading:18368410-Aged,
pubmed-meshheading:18368410-Cells, Cultured,
pubmed-meshheading:18368410-Culture Media, Serum-Free,
pubmed-meshheading:18368410-Cyclooxygenase 2,
pubmed-meshheading:18368410-Cyclooxygenase Inhibitors,
pubmed-meshheading:18368410-Dinoprostone,
pubmed-meshheading:18368410-Drug Interactions,
pubmed-meshheading:18368410-Humans,
pubmed-meshheading:18368410-Indoles,
pubmed-meshheading:18368410-Interleukin-1beta,
pubmed-meshheading:18368410-Isoxazoles,
pubmed-meshheading:18368410-Ketanserin,
pubmed-meshheading:18368410-Leukotriene B4,
pubmed-meshheading:18368410-Macrophages,
pubmed-meshheading:18368410-Middle Aged,
pubmed-meshheading:18368410-Osteoarthritis,
pubmed-meshheading:18368410-RNA, Messenger,
pubmed-meshheading:18368410-Receptor, Serotonin, 5-HT2A,
pubmed-meshheading:18368410-Receptors, Serotonin, 5-HT3,
pubmed-meshheading:18368410-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18368410-Serotonin,
pubmed-meshheading:18368410-Serotonin Agents,
pubmed-meshheading:18368410-Serotonin Antagonists,
pubmed-meshheading:18368410-Synovial Membrane,
pubmed-meshheading:18368410-Tumor Necrosis Factor-alpha
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pubmed:year |
2008
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pubmed:articleTitle |
Serotonin mediates PGE2 overexpression through 5-HT2A and 5-HT3 receptor subtypes in serum-free tissue culture of macrophage-like synovial cells.
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pubmed:affiliation |
Rheumatology, Medizinische Universitäts-Poliklink, Bonn, Germany. Matthias.Seidel@ukb.uni-bonn.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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