Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2008-5-15
pubmed:abstractText
Abelson murine leukemia virus (Ab-MLV) arose from a recombination between gag sequences in Moloney MLV (Mo-MLV) and the c-abl proto-oncogene. The v-Abl oncoprotein encoded by Ab-MLV contains MA, p12, and a portion of CA sequences derived from the gag gene of Mo-MLV. Previous studies indicated that alteration of MA sequences affects the biology of Mo-MLV and Ab-MLV. To understand the role of these sequences in Ab-MLV transformation more fully, alanine substitution mutants that affect Mo-MLV replication were examined in the context of Ab-MLV. Mutations affecting Mo-MLV replication decreased transformation, while alanine mutations in residues dispensable for Mo-MLV replication did not. The altered v-Abl proteins displayed aberrant subcellular localization that correlated to transformation defects. Immunofluorescent analyses suggested that aberrant trafficking of the altered proteins and improper interaction with components of the cytoskeleton were involved in the phenotype. Similar defects in localization were observed when the Gag moiety containing these mutations was expressed in the absence of abl-derived sequences. These results indicate that MA sequences within the Gag moiety of the v-Abl protein contribute to proper localization by playing a dominant role in trafficking of the v-Abl molecule.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-10373409, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-10514006, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-10559369, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-10729146, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-11160680, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-12467570, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-12476303, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-12663768, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-12776176, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-1370711, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-1549131, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-15681433, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-16227978, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-163444, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-16628219, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-1696637, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-17596313, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-17707624, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-1942244, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-2005881, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-2828693, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-2852893, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-2983109, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-3005619, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-6193890, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-7807010, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-7834738, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-8551558, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-8612582, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-8617726, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-9188629, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-9658142, http://linkedlifedata.com/resource/pubmed/commentcorrection/18367522-9789064
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1098-5514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5307-15
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:18367522-Abelson murine leukemia virus, pubmed-meshheading:18367522-Amino Acid Sequence, pubmed-meshheading:18367522-Binding Sites, pubmed-meshheading:18367522-Crystallography, X-Ray, pubmed-meshheading:18367522-Dimerization, pubmed-meshheading:18367522-Gene Products, gag, pubmed-meshheading:18367522-Models, Molecular, pubmed-meshheading:18367522-Molecular Sequence Data, pubmed-meshheading:18367522-Moloney murine leukemia virus, pubmed-meshheading:18367522-Mutation, pubmed-meshheading:18367522-Oligosaccharides, pubmed-meshheading:18367522-Oncogene Proteins v-abl, pubmed-meshheading:18367522-Peptides, pubmed-meshheading:18367522-Protein Structure, Quaternary, pubmed-meshheading:18367522-Protein Structure, Tertiary, pubmed-meshheading:18367522-Sequence Alignment, pubmed-meshheading:18367522-Sequence Homology, Amino Acid
pubmed:year
2008
pubmed:articleTitle
Mutations affecting the MA portion of the v-Abl protein reveal a conserved role of Gag in Abelson murine leukemia virus (MLV) and Moloney MLV.
pubmed:affiliation
Molecular Microbiology Graduate Program, Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts 02111, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural