Linked Life Data

18358820

Source:http://linkedlifedata.com/resource/pubmed/id/18358820

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-4-28
pubmed:abstractText
Skeletal muscle comprises several fiber types classified based on their contractile and metabolic properties. Skeletal muscle fiber types are classified according to their myosin heavy chain isoforms (MyHC I, IIa, IIx, and IIb). We attained good separation of MyHC isoforms in a mini-gel system by modifying a previously developed electrophoresis protocol. Increased glycerol and decreased cross-linking agent concentrations improved the separation of MyHC isoforms. Sample preparation with dithiothreitol and protease inhibitors produced clear MyHC band boundaries. This protocol included silver staining, with a linear range. The protocol provided high resolution and a highly accurate assay of rodent MyHC isoforms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1096-0309
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
377
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
111-3
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Protocol for high-resolution separation of rodent myosin heavy chain isoforms in a mini-gel electrophoresis system.
pubmed:affiliation
Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan. wataru_m@agr.kyushu-u.ac.jp
pubmed:publicationType
Journal Article